![]() ![]() Graphics & Metaphors Do you want to illustrate such concepts as growth, goal-reaching, leadership, or success with accurate visual metaphors? Our free graphics and metaphors for PowerPoint and Google Slides are here to help you grab your audience’s attention.Lay your ideas on the table and enjoy our free Text and Tables slide templates for PowerPoint and Google Slides! ![]() If you are looking for more specific content, you will find it here too: templates for your quotes and tables of contents, for instance.If you want to display your budget, invoices, and prices, plan your tasks, compare and contrast data or ideas, a great variety of tables is available to you.
0 Comments
![]() ![]() 2 C-terminally tagged proteins require a different procedure and are not studied here). Using these components, we established a standard protocol to visualize proteins with N-terminal CnTags (Fig. These could be obtained by routine cloning and purification procedures because the start-methionine preceding the CnTag (PGA.) is removed during protein expression by endogenous methionine aminopeptidase 16, 17. Furthermore, we generated target proteins with N-terminal CnTags (PGAFDADPLVVEI) and short linker sequences (5 amino acids, e.g., AAAGA (see Supplementary Information)). We designed a peptide substrate, with the Cy5.5 fluorophore at the N-terminus, followed by 20 residues based on the Methanosarcina mazei MtrA sequence (Cy5.5-RELAS KDPGAFDADPLVVEI). coli cell extract to simulate sample impurities. Sensitive and reliable target protein detectionįor our experiments, we used Connectase from Methanosarcina mazei and performed all reactions at room temperature, neutral pH (7.0–7.5), moderate salt concentrations (150 mM NaCl, 50 mM KCl), and in presence of E. Consequently, an alternative CnTag-substrate can replace the original CnTag-fragment in the reverse reaction, resulting in the formation of a new fusion product (Fig. This reaction is reversible, meaning that the substrate is constantly cleaved and re-ligated. In other words, the N-terminal substrate fragment ending in KD is fused to the enzyme (in the following N-Cnt), while the C-terminal fragment starting with PGA plus another 10 amino acids (in the following CnTag) is cleaved off. Upon binding, Connectase cleaves the amide bond between aspartate and proline in the KDPGA sequence and forms a new amide bond between its own N-terminal amino group and said aspartate. We found that Connectase acts on this recognition sequence, even when it is fused to other proteins or molecules. This sequence consists of a highly conserved KDPGA motif and 10 residues C-terminal of that motif, which vary across different species. Here, it recognizes a sequence in its interaction partner Methyltransferase A (MtrA), which links the catalytic and the transmembrane domain. This enzyme, Connectase (Cnt), is found in methanogenic archaea. Recently, we identified a protein ligase with very different characteristics 16. Remarkably, however, no protein ligase-based system has yet been established, primarily because the few known ligase enzymes have a low substrate specificity and suffer from side reactions 15. Alternatively, His 6- 13 or His 12-tags 14 can be detected directly in polyacrylamide gels by fluorophore-conjugated chelator probes. For example, a C-terminal 11 amino acid fragment, the HiBiT tag, can be used to reconstitute a split luciferase in order to visualize the tagged protein on a blotting membrane 12. Similar small protein tags can also be detected by other means. Popular tags include Streptavidin-, FLAG-, V5-, cMyc-, His 6-, HA-, or E-tags, and vary between 6–14 amino acids in length, plus additional linker sequences. As these antibodies are often better characterized and more reliable compared to POI-specific antibodies 8, many researchers prefer this method to study exogenous recombinant proteins, and an increasing number of scientists use gene-editing tools to insert tags in endogenous proteins 9, 10, 11. ![]() In such cases, a small peptide tag can be introduced, which is detectable with a tag-specific antibody. While commercial POI-specific antibodies are available for many proteins from model organisms and for well-studied targets, no suitable antibodies are on the market for the majority of proteins. In addition, quantitative analyses are complicated 4 and often result in a hyperbolic rather than in the desired linear signal-to-substrate relationship 1, 4, 7. Even with identical samples and standard operating procedures, results from different users may vary significantly 6. This impedes the comparability of different blots, in particular across different labs 3, 4, 5. Overall, the procedure involves multiple steps, which are often adapted depending on the target protein, the chosen antibody combination, or the sample composition 1, 2. After additional binding sites on this membrane have been blocked, target-specific antibodies are used to detect the protein of interest (POI), and these, in turn, are visualized by reporter-conjugated antibodies. Here, proteins within a complex mixture are separated on a polyacrylamide gel and transferred to a membrane. Western blots present one of the most widely used methods in molecular research. ![]() ![]() ![]() Many text art generators will allow you to save your design as an image file or share it directly on social media or messaging apps. Save or share: Once you're satisfied with your design, you can save it or share it with others. Preview and adjust: Preview your design and make any necessary adjustments, such as changing the color or size of the characters or adding additional lines or shapes.ĥ. Input your design: Use the text art generator's tools to input your design, such as selecting characters and symbols, adjusting line thickness and spacing, and choosing a font.Ĥ. Select the type of text art: Choose the type of single line text art you want to create, such as ASCII art, emoji art, symbol art, letter art, or one-line drawings.ģ. Choose one that you find user-friendly and meets your needs.Ģ. Choose a text art generator: There are many online text art generators available, both free and paid. Here are the steps to create single line text art using a text art generator:ġ. How to make single line text art by using text art generator ? Personal expression: funny ascii art can be used as a form of self-expression and a way to share one's creativity with others. Printing and packaging: one liner text art can be used to create attractive and distinctive designs for product packaging, posters, and other printed materials.Ħ. Web design: small ascii art can be used to create eye-catching graphics and illustrations for websites and other digital media.ĥ. Logos and branding: one line ascii art can be used to create unique and memorable logos and branding materials.Ĥ. Text-based messaging apps: ascii art single line can be used to express an idea or emotion in a text message, chat, or email.ģ. Social media: small text art can be used as an attention-grabbing post or as a way to express an idea or emotion.Ģ. Single line text art can be used in a variety of contexts, including:ġ. Where you can use these single line text art ? One-Line Drawings: One-line drawings are continuous line drawings that depict a subject in a single, uninterrupted line.Įach type of single line text art has its own unique style and creative possibilities, and some artists combine different techniques to create unique and intricate designs. Letter Art: Letter art is created using letters and characters to represent images and designs.ĥ. Symbol Art: Symbol art is created using special characters and symbols to represent images and designs.Ĥ. Emoji Art: Emoji art is created using emoji characters to represent images and designs.ģ. ASCII Art: ASCII art is created using the ASCII character set to represent images and designs.Ģ. There are several types of single line text art, including:ġ. The goal is to create the illusion of a picture or design with a single, unbroken line of ASCII characters. The characters are arranged in a way that they form a recognizable shape or pattern, but with a continuous single line with no breaks or spaces. Single line ASCII art refers to the creation of images, logos, or designs using ASCII characters (i.e., the standardized character encoding used for electronic communication). The goal is to create the illusion of a picture or design with a single, unbroken line of text characters. Single line text art refers to the creation of images, logos, or designs using characters, symbols, and letters, arranged in a way that they form a recognizable shape or pattern, but with a continuous single line with no breaks or spaces. Single line art copy paste now! What is mean by single line text art ? ![]() So, what are you waiting for? Go ahead and The side bar gives you a quick access toġ00+ different categories and saves your time. To make navigation easy you can utilize this sidebar. ![]() We created this single line keyboard art website because we needed a easy way to create and copy cool single line text art One line text art, 1 line ascii art, ascii art one line and many more. It includes ascii art single line, one line ascii, single line ascii art, Here you get a large collection of single line text and single line ascii art, around 100+ single line art at single ![]() ![]() You can download and install Service Pack 1 for Office 2013 from the following Microsoft Knowledge Base article:Ģ817430 Description of Microsoft Office 2013 Service Pack 1 (SP1)ģ141466 January 3, 2017, update for Outlook 2013 (KB3141466)ģ115404 August 2, 2016, update for Outlook 2013 Junk Email Filter (KB3115404)ģ054854 June 9, 2015, update for Outlook 2013 (KB3054854) Updates for Office 2013 programs that are dated April 14, 2015, or later require Office 2013 Service Pack 1 (SP1) to be installed before you can install the update. You cannot install the updates from these articles on click-to-run installations of Office 2013. By default, click-to-run installations are automatically updated. They do not apply to Click-to-run installations of Office 2013. The following updates apply only to Office 2013 clients that are installed by using a. You cannot install the updates from these articles on click-to-run installations of Office 2016.ģ141453 January 3, 2017, update for Outlook 2016 (KB3141453)ģ115407 August 2, 2016, update for Outlook 2016 Junk Email Filter (KB3115407)ģ128057 MS17-002: Description of the security update for Word 2016: January 10, 2017ģ141473 January 3, 2017, update for Office 2016 (KB3141473)Īfter you install all these updates, you should see the following versions of important files that are used by Outlook. They do not apply to Click-to-run installations of Office 2016. The following updates apply only to Office 2016 clients that are installed by using a.
![]() ![]() Fitness Extension Pack – This one is great if you want to track your food and water intake and your exercise goals.I use separate Expense Tracker pages to track my blog’s income and expenses and our family’s recurring monthly bills. Budget Extension Pack – This is the one I have, and I LOVE it! It’s got space to track all of your monthly expenses and budget.Here’s a picture of the three Happy Planner sizes for reference: I actually don’t think that there is much difference in the box sizes on the calendar pages from the Classic to the Big size. I have the Classic size and it suits my needs just fine. The Mini Happy Planner – If you want a purse-sized planner that’s still cute, then this one is for you! The Mini Happy Planner pages are size 4.5″ x 7″, and the cover measures 5.125″ x 7.5″.It seems to me that there are WAY more accessories available for the Classic size. The Classic Happy Planner – The pages of the Classic Happy Planner are size 7″ x 9.25″ and the cover measures 7.75″ x 9.75″.The nice thing about the Big Happy Planner is that you can easily add your own printables without having to go through the hassle of resizing them. ![]() The Big Happy Planner – The pages of the Big Happy Planner are the standard 8.5″ x 11″, and the cover size is 9″ x 11.25″.What sizes does The Happy Planner come in? I have the gold metal classic size discs, and I LOVE them, though they are a bit pricier than the plastic option. The discs that hold the planner together not only look BEAUTIFUL, they’re also extremely functional and brilliant!Īnd the discs are just one example of how the makers of the Happy Planner are super detail-oriented… notice the cute heart in the center: Happy Planners have a nifty disc binding that’s kind of like a cross between a 3-ring binder and a spiral notebook. That’s what sets the Happy Planner apart from your normal planners. The Happy Plannerĭid you notice the part about the wildly popular planners being “disc-bound”? The Happy Planner is a disc-bound planner system that combines your love for Creativity with your need for Organization. The Happy Planner (sometimes referred to as the Create 365 Planner or the MAMBI Planner) is made by Me and My Big Ideas. ![]() Read my full disclosure here.įirst off, let me answer some common questions from Happy Planner beginners: FAQs from Happy Planner Beginners: Who makes The Happy Planner? *This post contains affiliate links, which means I earn a commission from these links if you make a purchase, at no additional cost to you. If you just jumped on the Happy Planner bandwagon, then here is everything you need to know as a Happy Planner beginner. Heck, yes I want to bling-up my planner with some fringe tassels!īut beyond the cute add-ons, the Happy Planner (sometimes called the Create 365 Planner) is actually REALLY well designed and super useful! I had been tempted by the long aisles of cute planner accessories from Happy Planner, and when it came time to buy another planner, I caved to the cute stickers, washi tape, and planner charms. ![]() ![]() ![]() Individual income tax rates now range from 1 to 6.5, and the number of tax brackets has been reduced from seven to five. Tax Rate Reduction Effective January 1, 2021, all tax rates have been decreased. Idaho doesn’t conform to bonus depreciation for assets acquired after 2009. The top marginal income tax rate of 37 percent will hit. Idaho conforms to the IRC as of January 1, 2021. There are seven federal income tax rates in 2023: 10 percent, 12 percent, 22 percent, 24 percent, 32 percent, 35 percent, and 37 percent. also maintains an archive of important Idaho income tax exemption amounts, including the Idaho Standard Deduction, the Idaho Personal Exemption, and the Idaho Dependent Deduction. The income limits for all 2023 tax brackets and all filers will be adjusted for inflation and will be as follows (Table 1). Here’s the Fall 2021 issue of Tax Update our newsletter for the business community. 2017 Idaho Tax Deduction Amounts also maintains an archive of important Idaho income tax exemption amounts, including the Idaho Standard Deduction, the Idaho Personal Exemption, and the Idaho Dependent Deduction. Income tax brackets are required state taxes in Idaho based on relative income and earnings. This means that these brackets applied to all income earned in 2022, and the tax return that uses these tax rates was due in April 2023.īoth Idaho's tax brackets and the associated tax rates were last changed one year ago in 2022. The latest available tax rates are for 2023, and the Idaho income tax brackets have been changed since 2016. The tax rates are broken down into groups called tax brackets. This page shows 's archived Idaho tax brackets for tax year 2022. House Bill 172 Effective January 1, 2023. ![]() For individuals, the rate applies to taxable income over: 4,489 if you’re filing single. ![]() FREE for simple returns, with discounts available for users! Idaho’s tax rate is now 5.8 for all taxpayers. File your Idaho and Federal tax returns online with TurboTax in minutes. EFiling is easier, faster, and safer than filling out paper tax forms. ![]() ![]() ![]() That will be that "Index" number that was assigned to each sensor element when you configured HWiNFO. ValueRaw N : This will contain a raw, unformatted version of the number value.Value N : This will contain a formatted version of the number value.įormatting will include commas for thousands separators, and the text value for the unit of measure.Label N : This will contain the name of the individual sensor element.Sensor N : This will contain the name of the top level sensor.You don't want HWiNFO using more resources than it needs to.įor each sensor element, four registry entries will be created and updated. This can be changed in the settings for HWiNFO, but I don't particularly recommend changing it. This will be done each time HWiNFO "polls" your sensors, which by default is every 2000ms or every 2 seconds. What happens is that HWiNFO will output the current values for all of the sensor elements you selected to the Windows Registry. The hard work is behind you! Using the values in a skin is actually really simple. HWiNFO will minimize to the Windows Notification Area, and will continue to run in the background. Note that Shared Memory Support is not required and should be left turned off:Ĭlick on "OK" and you are done! You can close the Sensor Status dialog. Then using the "Settings" option from the Windows Notification Area tray icon, configure HWiNFO with these recommended options. The first thing will be to get version 7.02 or later of the software from HWiNFO Home. The end-user of a skin will need to do some setup work with HWiNFO before using the skin. A distinct HWiNFO plugin for Rainmeter is no longer required. This allows you to output the current values of one or more sensors to the Windows Registry, where it can be easily accessed using the Registry measure in Rainmeter. ![]() The approach consists of tweaking a feature of HWiNFO that was implemented years ago to support using the software with the now long-dead Windows Sidebar gadgets. Martin Malik, the author of HWiNFO, has graciously worked with us to find a solution that will allow the software to be used for personal, non-commercial use in Rainmeter without having to purchase a license, while keeping his ability to monetize his application when used by commercial entities. This would make distributing Rainmeter skins to end-users problematic at best. rmskin fileĥ.As of version 7.0 of the HWiNFO hardware sensor monitoring tool, the software has changed to require purchasing a yearly "Pro" license in order to use the Shared Memory feature of the software that allowed it to interact with the HWiNFO plugin for Rainmeter. Make sure you have downloaded the "OpenHardwareMonitorPlugin.dll". Copy exactly how it is written in Open Hardware MonitorĤ. Replace CPU and GPU name variables with your own, under the VARIABLES tab. I personally also turn on "Start Minimized", "Start to Tray", "Minimize on Close"ģ. In Open Hardware Monitor "Options", set "Run on Windows Startup". Have Open Hardware Monitor downloaded prior to loading skin (Made with version 0.9.6)Ģ. If these authors or any users have any issues or suggestions with my adaptation, please get in contactġ. ini file for setup guide.Īdapted from Meddx's "Dashboard" and Patrick Stillhart's "Spinner". Created with version 0.9.6 but I assume it should work with other versions. It uses Open Hardware Monitor as the source for the data so make sure you install it. Its my first rainmeter creation so let me know if you encounter any issues. Hey all, I've made a small widget style skin for background monitoring of CPU and GPU temperature, usage, and clock speeds. ![]() ![]() ![]() 10 months agoġ0 months ago I found a post from 2016 that instructs you on how to bypass getting the second lot 192 by altering ini files and using ghost walk to get into the elvator. BTW I am also on windows 11 so other versions of windows may have the user configuration file somewhere else. Hope this helps anyone that got stuck like I did. The post also mentions that you will have to repurchase all your plasmid slots after doing this. As the post suggests you must approach the elevator from behind to enter it and once you do hit your walk key and Bob's your uncle. Next, save the user file and start the game. You need the Walk command to disable Ghost. I used F12 for Ghost (which is the correct command for ghost walking and I used F11 for Walk (also the correct command). It will open a large file but don't fret just scroll down a short distance until you see all the key bindings for F1-F12. From there click on the User file type for configuration settings. This is the post *LINK REMOVED* I have the game on steam so my user ini file was located here C:\Users\"yourname"\Appdata\BioshockHD\Bioshock. TextureStreamingDistanceLimit=30000.Dazowx: I found a post from 2016 that instructs you on how to bypass getting the second lot 192 by altering ini files and using ghost walk to get into the elvator. So here's how my Bioshock2SP.ini looks like: I made saves once in a while just to be sure, but I don't think it will be necessary anymore. First I thought I only got lucky, but now I'm playing Minerva's Den and still no crash. I played through Fontaine Futuristics right until I finished the whole game in Persephone and it didn't crash once. However, now all of a sudden it seems to run rather smooth and didn't crash in a while. I tried using the methode above, updated my graphic driver, re-installed DirectX and vcredist, tried other methodes and nothing worked. TextureStreamingDistanceLimit=30000.000000 OffsetSkeletalProjectorRearFrustrumPlane=10.000000ĬascadingWaterSimulationVelocity=10.000000 Also the same can be done for the remaster of Bioshock 1. If anyone else has any other insight into any of the other settings let me know. This fix not only fixed my crashing but also made loading/save times shorter. ![]() If you change any settings in game after you make the changes to the ini, it will revert back and the crashes will start again. After you made those changes save and exit. I will post what mine looks like at the end of the post. There are also some other settings here that you can change to get better effects. Also find UseMultithreadedRendering and change it to UseMultithreadedRendering=True. Under find UseMultithreading and change it to UseMultithreading=True. Navigate to C:\Users\username\AppData\Roaming\BioshockHD\Bioshock2 (Replace username with the user name you chose for windows) open the file Bioshock2SP.ini with either notepad or notepad++(I prefer notepad++) Navigate to. ![]() Also turn off VSync in game(if you are like me and hate screen tare you can add a profile in your GFXs card's control panel like I do, just make sure to also enable triple buffering). Now before you do ANYTHING go into game, set all the settings/keybindings/mouse settings to where you want them because if you change ANYTHING and hit apply the settings you change here will revert back. ![]() ![]() ![]() Cheap fibre supplement can improve your memory in just 12 weeks: Scientists say they are 'excited' by discovery.Rishi Sunak roasts SNP for turning Scotland into the 'high-tax capital of the UK' as he slams their 'obsession' with splitting the union in speech to Tory conference in Aberdeen.Full details of explosive court case in which two women falsely said Brigitte Macron was born a man and 'ultra-protected people' were covering it up are revealed after her daughter revives rumours.Met Office confirms last month was the hottest February on RECORD in England and Wales - and one of the wettest in history.Speeding car smashes into pedestrians crossing road in Poland, injuring 17 people including three children.The real killer of The Trial exposed… and the defenceless wife he bludgeoned to death as featured in gripping Channel 4 jury experiment.What's behind Meghan and Harry's royal-style PR blitz? Experts say Sussexes' new website, awards ceremonies and engagements are part of efforts to move on from royal bombshell damage.Martinis before church, William Blake and Vaughan Williams for tears and then meat and potato pie and chips at the wake: The poignant funeral wishes Dave Myers revealed before his death. ![]() BORIS JOHNSON: It's time for tobacco-style warnings on UPF food, with pictures of the giant pot belly it can give you.Meanwhile, staff are ordered to let thieves take what they want. Inside shoplifting Ground Zero: Gangs ransack stores with impunity every day while woke police do nothing.'This has gone on long enough': Rishi Sunak pleads for calm and urges Britain to unite against extremism after 'alarming' win for George Galloway in by-election and months of street protests across the UK.Christian Horner's accuser hits back amid 'leaked texts' scandal: Female employee says she is 'very unhappy' at Red Bull's decision to clear F1 boss of inappropriate behaviour and slams 'one-sided' probe into allegations.Only people with high IQs can spot the odd letter in this brain teaser in under 13 seconds.The 'best news' Fergie 'could have hoped for': 'Huge relief' for Duchess of York as she is told there appears to be no spread of her skin cancer.but it could be too late to stop the ultimate nightmare NIALL FERGUSON: Europeans are finally waking up from their defenceless fool's paradise.The grubby texts 'from boss Christian Horner to subordinate female employee': From 'Santa PJ selfies' to 'stretching legs' and jokes about Spanx, the tacky message exchange being shared on social media as F1 boss returns to the paddock in Bahrain.Geri Halliwell's show of solidarity amid leaked text scandal: Spice Girl lands in Bahrain on private jet to meet husband Christian Horner - as Red Bull F1 chief is hauled in for crisis talks.Lady Gabriella Windsor's husband Thomas Kingston, 45, died from a 'traumatic head wound' and a gun was found near his body in outbuilding of his parents' Cotswolds home, inquest hears. ![]() ![]() A Discord Server List such as Discadia is a place where you can advertise your server and browse servers promoted by relevance, quality, member count, and more. What is a Discord Server List?Ī Discord Server List is a website that provides a way for you to find the Discord servers that you're looking for. Discadia uses a proprietary algorithm to deliver you the best Discord Servers that you're likely to be interested in. Using Discadia you can browse through thousands of servers, search, and filter by tags. While there's a huge range of Discord servers out there, not all of them may appeal to you.
|
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |